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Antigens which detect IgE antibodies in workers sensitive to toluene diisocyanate
Author(s) -
KAROL MERYL H.,
ALARIE YVES
Publication year - 1980
Publication title -
clinical and experimental allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.462
H-Index - 154
eISSN - 1365-2222
pISSN - 0954-7894
DOI - 10.1111/j.1365-2222.1980.tb02086.x
Subject(s) - isocyanate , antigen , toluene diisocyanate , antibody , immunoglobulin e , human serum albumin , chemistry , toluene , serum albumin , picryl chloride , monoclonal antibody , albumin , immunology , human albumin , medicine , biochemistry , polyurethane , organic chemistry
Summary Two antigens, both containing tolyl groups, were compared for ability to detect IgE antibodies in workers hypersensitive to toluene diisocyanate. One antigen, formed by reaction of p‐tolyl isocyanate with human serum albumin, detected antibodies in each of ten hypersensitive workers. A second tolyl antigen, formed by reaction of p‐toluoyl chloride and human serum albumin, and therefore lacking isocyanate linkages, detected antibodies in seven of the ten workers. In RAST assays, sera from toluene diisocyanate‐sensitive workers demonstrated higher binding to human serum albumin‐coated control discs than did sera from non‐sensitive workers. The significance of this binding in calculating tolyl‐reactive antibody titres is discussed.