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The use of radioactive isotopes for analysis of cutaneous allergic responses
Author(s) -
JAN K.,
NAHMIAS C.,
COATES G.,
HARGREAVE F. E.,
DAVIS C.,
DOLOVICH J.
Publication year - 1980
Publication title -
clinical and experimental allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.462
H-Index - 154
eISSN - 1365-2222
pISSN - 0954-7894
DOI - 10.1111/j.1365-2222.1980.tb02076.x
Subject(s) - histamine , chemistry , allergic reaction , skin reaction , in vivo , allergy , medicine , radiochemistry , immunology , biology , microbiology and biotechnology
Summary Local exudation at the sites of allergic and histamine‐induced cutaneous reactions, was measured with an in vivo double‐labelling procedure, based upon local serial simultaneous measurements of indium‐113 m ( 113m In) labelled transferrin and technitium‐99m labelled red blood cells ( 99m Tc RBC). Analyses depended upon the reaction index i.e. comparison of the ratio of 113m In/ 99m Tc counts at the reaction site with the ratio of 113m In/ 99m Tc at the corresponding unreacted site in the skin of the opposite arm. Reaction indices were initially significantly elevated at the sites of histamine and allergic reactions. After the first hour, there was a progressive reduction at histamine sites indicating a tapering of the local accumulation of indium‐labelled transferrin. By contrast allergic reaction sites showed a continuing increase in the reaction index after one hour. During the first hour the curves were comparable, but they diverge and were significantly different after one hour (0.05 > P > 0.02). This is consistent with the known occurrence of late cutaneous responses at the site of allergic but not histamine reactions.