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Specific IgE antibodies to platinum salts in sensitized workers
Author(s) -
CROMWELL O.,
PEPYS J.,
PARISH W. E.,
HUGHES E. G.
Publication year - 1979
Publication title -
clinical and experimental allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.462
H-Index - 154
eISSN - 1365-2222
pISSN - 0954-7894
DOI - 10.1111/j.1365-2222.1979.tb01529.x
Subject(s) - immunoglobulin e , conjugate , cyanogen bromide , sepharose , chemistry , platinum , antibody , salt (chemistry) , covalent bond , immunology , biochemistry , chromatography , medicine , organic chemistry , enzyme , catalysis , peptide sequence , mathematics , mathematical analysis , gene
Summary A RAST has been developed for the measurement of IgE antibodies specific to platinum chloride complexes in sensitized workers. Human serum albumin covalently linked to Sepharose beads by the cyanogen bromide method was reacted with ammonium tetrachloroplatinite (II) (NH 4 ) 2 PtCl 4 . This conjugate was more suitable for the RAST, than conjugates of HSA and the platinum salt prepared in solution and then linked to the activated Sepharose, showing better sensitivity and giving lower levels of non‐specific uptake of IgE from sera of non‐exposed subjects with high total levels of IgE, e.g. allergic bronchopulmonary aspergillotics.