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Allergic drug reactions: an in vitro model using a mixed function oxidase complex to demonstrate antibodies with specificity for a practolol metabolite
Author(s) -
AMOS H. E.,
LAKE B. G.,
ATKINSON H. A. C.
Publication year - 1977
Publication title -
clinical and experimental allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.462
H-Index - 154
eISSN - 1365-2222
pISSN - 0954-7894
DOI - 10.1111/j.1365-2222.1977.tb01473.x
Subject(s) - practolol , metabolite , mixed function oxidase , drug , in vitro , antibody , chemistry , pharmacology , drug allergy , immunology , medicine , biochemistry , microsome , propranolol
Summary Reactive metabolite products of the drug practolol were generated in vitro using the rat liver mixed function oxidase complex. The metabolites were spontaneously coupled to a non‐agglutinating rabbit antibody to human O red blood cells, so forming a metabolite–antibody reagent. This reagent was then used to passively sensitize human O red cells which subsequently acted as indicator cells for detecting anti‐metabolite antibodies.