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The inhibitory effect of serum factors on measurement of IgE aspergillus antibodies by RAST
Author(s) -
VERVLOET DANIEL,
FUJITA YOSUKE,
WYPYCH JOHN I.,
REISMAN ROBERT E.,
ARBESMAN CARL E.
Publication year - 1974
Publication title -
clinical and experimental allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.462
H-Index - 154
eISSN - 1365-2222
pISSN - 0954-7894
DOI - 10.1111/j.1365-2222.1974.tb01397.x
Subject(s) - immunoglobulin e , aspergillus fumigatus , radioallergosorbent test , blocking antibody , immunology , antibody , allergen , chemistry , aspergillus , allergic bronchopulmonary aspergillosis , allergy , medicine , microbiology and biotechnology , biology
Summary The RAST value obtained in the A. fumigalus system, from sera of patients with allergic bronchopulmonary aspergillosis (ABPA) is dependent not only on the reagin titre but also on blocking factors that depress the RAST score. These factors are primarily specific for A. fumigatus and can be removed by an A. fumigatus immuno‐adsorbent. However, some blocking activity could be found in normal sera even after absorption with an A, fumigates immunoadsorbent and seems to be non‐specific. All of these blocking factors influence the specificity of the RAST, and complicate the comparison of the quantitation of IgE antibodies in different sera. The use of discs rather than cellulose particles and the use of dilute sera result in a RAST assay more proportional to the specific IgE antibodies to A. fumigates .