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Photo‐inactivated allergens I. Preparation, physicochemical and biological properties
Author(s) -
BERRENS L.,
HÉNOCQ E.,
RADERMECKER M.
Publication year - 1973
Publication title -
clinical and experimental allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.462
H-Index - 154
eISSN - 1365-2222
pISSN - 0954-7894
DOI - 10.1111/j.1365-2222.1973.tb01352.x
Subject(s) - in vitro , chemistry , allergen , in vivo , lysine , absorption (acoustics) , ultraviolet light , antibody , antigen , immunology , allergy , biochemistry , medicine , biology , photochemistry , amino acid , microbiology and biotechnology , materials science , composite material
Summary A method is described for the preparation of inactive atopic allergens by irradiation with ultraviolet light. From an analysis of the absorption and fluorescence emission spectra, and with the aid of synthetic model compounds, it is concluded that the allergenic determinant of lysine‐sugar conjugation is extremely sensitive to ultraviolet light. Considerable reduction of allergenicity in vitro (diminished capacity to inactivate human complement in fluid phase) and in vivo (impressive reduction of skin‐reactivity in atopic patients and, by passive transfer, in monkeys) was demonstrated. Only slight modification of the antigenic characteristics was detected.