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Basophil leucocyte separation from human peripheral blood: a technique for their isolation in high purity and high yield
Author(s) -
DAY R. P.
Publication year - 1972
Publication title -
clinical and experimental allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.462
H-Index - 154
eISSN - 1365-2222
pISSN - 0954-7894
DOI - 10.1111/j.1365-2222.1972.tb01284.x
Subject(s) - centrifugation , peripheral blood , yield (engineering) , basophil , chromatography , differential centrifugation , human blood , isolation (microbiology) , immunology , chemistry , medicine , materials science , biology , antibody , immunoglobulin e , composite material , biochemistry , physiology , microbiology and biotechnology
Summary A method is described whereby viable basophil leucocytes are isolated from human peripheral blood by density gradient centrifugation. The rate of recovery averaged 74% and the purity averaged 93% of the final preparation.