Open AccessComparison of cell proliferation and epigenetic modification of gene expression patterns in canine foetal fibroblasts and adipose tissue‐derived mesenchymal stem cells
Author(s) -
Oh H. J.,
Park E. J.,
Lee S. Y.,
Soh J. W.,
Kong I. S.,
Choi S. W.,
Ra J. C.,
Kang S. K.,
Lee B. C.
Publication year - 2012
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/j.1365-2184.2012.00838.x
Subject(s) - sox2 , mesenchymal stem cell , biology , epigenetics , somatic cell nuclear transfer , stem cell , cell growth , microbiology and biotechnology , adipose tissue , cell , embryonic stem cell , gene , embryo , genetics , embryogenesis , blastocyst , endocrinology
Objectives This study compared rate of cell proliferation, viability, cell size, expression patterns of genes related to pluripotency and epigenetic modification between canine foetal fibroblasts (c FF ) and canine adipose tissue‐derived mesenchymal stem cells (cAd‐ MSC ). Materials and methods Proliferation pattern, cell viability as well as cell size at each passage of c FF and cAd‐ MSC were measured when cultures reached confluence. In addition, real‐time PCR was performed to investigate expression of Dnmt1 , HDAC1 , OCT4 , SOX2 , BAX, BCL2 genes with reference to β‐actin gene expression as an endogenous control in both cell lines. Results c FF and cAd‐ MSC differed in number of generations, but not in doubling times, at all passages. Mean cell size of cAd‐ MSC was significantly smaller than that of c FF . Cell viability was significantly lower in c FF s and apoptotic level was significantly lower in cAd‐ MSC compared to passage‐matched c FF . In the expression of genes related to pluripotency and epigenetic modification, level of HDAC 1 in cAd‐ MSC was significantly higher than in c FF , but expression of Dnmt1 did not differ between the two groups. OCT4 and SOX2 were significantly more highly expressed in cAd‐ MSC compared to c FF . Conclusions cAd‐ MSC have higher stem‐cell potential than c FF in terms of proliferation patterns, epigenetic modification and pluripotency, thus cAd‐ MSC could be more appropriate than c FF as donors of nuclei in somatic cell nuclear transfer for transgenesis.