Open AccessS clerotium rolfsii lectin inhibits proliferation and induces apoptosis in human ovarian cancer cell line PA ‐1
Author(s) -
Eligar S. M.,
Pujari R.,
Swamy B. M.,
Shastry P.,
Inamdar S. R.
Publication year - 2012
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/j.1365-2184.2012.00831.x
Subject(s) - apoptosis , biology , population , sclerotium , microbiology and biotechnology , flow cytometry , cell growth , cell culture , annexin , caspase , programmed cell death , biochemistry , medicine , genetics , environmental health , horticulture
Objectives S clerotium rolfsii lectin ( SRL ), isolated from soil born phytopathogenic fungus S clerotium rolfsii, exhibits exquisite binding specificity to the oncofoetal Thomsen‐Friedenreich (Galβ1,3Gal NA cα‐ O ‐Ser/Thr, T or TF ) antigen and associated glycans. In the present study, we report anti‐proliferative activity of SRL and investigate underlying mechanisms of SRL ‐induced apoptosis, in the human ovarian cancer cell line PA ‐1. Materials and methods SRL ‐induced anti‐proliferative effects were determined using MTT assay and induction of apoptosis was determined by flow cytometry, confocal microscopy and western blot analysis. Results SRL inhibited population growth of PA ‐1 cells in a dose‐ and time‐dependent manner with maximum inhibition (71.3 ± 1.9%) occurring at concentration of 50 μg/ml after 72 h incubation. Observed effects of SRL could be blocked by competing glycoproteins, asialomucin, mucin and fetuin. Treatment with SRL resulted in increase in hypodiploid cell population as determine by cell cycle analysis. Increase in numbers of annexin V‐ PI positive cells, and cleavage of PARP confirmed apoptosis‐inducing activity of SRL . Involvement of caspases in SRL ‐mediated apoptosis was determined by cleavage of caspases‐3, ‐8 and ‐9 in a time‐dependent manner, thereby suggesting possible involvement of both intrinsic and extrinsic caspase‐dependent pathways. Conclusion The present study demonstrates anti‐proliferative and apoptosis‐inducing activity of SRL that can be exploited for potential application in ovarian cancer research.