Open AccessEquisetum arvense hydromethanolic extracts in bone tissue regeneration: in vitro osteoblastic modulation and antibacterial activity
Author(s) -
Bessa Pereira C.,
Gomes P. S.,
CostaRodrigues J.,
Almeida Palmas R.,
Vieira L.,
Ferraz M. P.,
Lopes M. A.,
Fernandes M. H.
Publication year - 2012
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/j.1365-2184.2012.00826.x
Subject(s) - staphylococcus aureus , in vitro , antibacterial activity , microbiology and biotechnology , chemistry , cell growth , biology , population , viability assay , bacteria , biochemistry , medicine , genetics , environmental health
Objectives E quisetum arvense preparations have long been used to promote bone healing. The aim of this work was to evaluate osteogenic and antibacterial effects of E . arvense hydromethanolic extracts. Materials and methods Dried aerial components of E . arvense were extracted using a mixture of methanol:water (1:1), for 26 days, yielding three extracts that were tested (10–1000 μg/ml) in human osteoblastic cells: E1, E2 and EM (a mixture of E1 and E2, 1:1). Cell cultures, performed on cell culture plates or over hydroxyapatite (HA) substrates, were assessed for osteoblastic markers. In addition, effects of the extracts on S taphylococcus aureus were addressed. Results Solution E1 caused increased viability/proliferation and ALP activity at 50–500 μg/ml, and deleterious effects at levels ≥1000 μg/ml. E2 inhibited cell proliferation at levels ≥500 μg/ml. EM presented a profile between those observed with E1 and E2. In addition, E1, E2 and EM, 10–1000 μg/ml, inhibited expansion of S . aureus . Furthermore, E1, tested in HA substrates colonized with osteoblastic cells, causing increase in cell population growth (10–100 μg/ml). E1 also exhibited antibacterial activity against S . aureus cultured over HA. Conclusions Results showed that E . arvense extracts elicited inductive effects on human osteoblasts while inhibiting activity of S . aureus , suggesting a potentially interesting profile regarding bone regeneration strategies.