Open AccessGSK 3 inhibitor‐ BIO regulates proliferation of female germline stem cells from the postnatal mouse ovary
Author(s) -
Hu Y.,
Bai Y.,
Chu Z.,
Wang J.,
Wang L.,
Yu M.,
Lian Z.,
Hua J.
Publication year - 2012
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/j.1365-2184.2012.00821.x
Subject(s) - biology , homeobox protein nanog , stem cell , sox2 , klf4 , microbiology and biotechnology , embryoid body , embryonic stem cell , germline , induced pluripotent stem cell , genetics , gene
Objective It is widely believed that in most female mammalian neonates, all germ cells enter meiosis to form the primary oocyte at the end of foetal development, and as a result, the postnatal mammalian ovary harbours only a limited supply of oocytes that cannot be regenerated. However, this idea has been challenged by the discovery of the existence of female germline stem cells ( FGSC s) in postnatal mammalian ovaries. Materials and Methods We have isolated ovarian GSC s from neonatal and adult mouse ovaries and expanded them in the same culture conditions as embryonic stem cells ( ESC s). Results LIF and BIO were beneficial for formation of FGSC colonies. BIO promoted proliferation of FGSC s through activation of β‐catenin and up‐regulation of E‐cadherin. The FGSC s formed compact round colonies with unclear borders, maintained ESC characteristics and alkaline phosphatase ( AP ) activity, expressing germ‐cell markers‐Vasa, and stem‐cell markers: Oct4, Klf4, C‐myc, Nanog, CD 49f, Sox2, CD 133, SSEA 1 and SSEA 4. These cells had the ability to form embryoid bodies ( EB s), which expressed specific markers for all three germ layers. Then we induced EB s to differentiate into neurons, cardiomyocytes, pancreatic cells and germ cells, which showed the expression of specific markers, β‐III‐tubulin, cardiac a‐actin, Pdx1 and Zps respectively. Discussion and Conclusion This study reveals the existence of FGSC s in postnatal mouse ovary with multipotent characteristics. BIO played an important role in regulation of proliferation and maintenance of the FGSC s. This could help provide a better understanding of causes of ovarian infertility, prevention and potential treatment of infertility.