Open AccessPre‐culturing human adipose tissue mesenchymal stem cells under hypoxia increases their adipogenic and osteogenic differentiation potentials
Author(s) -
Valorani M. G.,
Montelatici E.,
Germani A.,
Biddle A.,
D'Alessandro D.,
Strollo R.,
Patrizi M. P.,
Lazzari L.,
Nye E.,
Otto W. R.,
Pozzilli P.,
Alison M. R.
Publication year - 2012
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/j.1365-2184.2012.00817.x
Subject(s) - mesenchymal stem cell , adipogenesis , microbiology and biotechnology , adipose tissue , progenitor cell , viability assay , stem cell , biology , hypoxia (environmental) , cellular differentiation , immunology , cell , andrology , chemistry , endocrinology , medicine , biochemistry , oxygen , organic chemistry , gene
Objectives Hypoxia is an important factor in many aspects of stem‐cell biology including their viability, proliferation, differentiation and migration. We evaluated whether low oxygen level (2%) affected human adipose tissue mesenchymal stem‐cell (h AT ‐ MSC ) phenotype, population growth, viability, apoptosis, necrosis and their adipogenic and osteogenic differentiation potential. Materials and methods h AT ‐ MSC s from four human donors were cultured in growth medium under either normoxic or hypoxic conditions for 7 days and were then transferred to normoxic conditions to study their differentiation potential. Results Hypoxia enhanced h AT ‐ MSC expansion and viability, whereas expression of mesenchymal markers such as CD 90, CD 73 and endothelial progenitor cell marker CD 34, remained unchanged. We also found that pre‐culturing h AT ‐ MSC s under hypoxia resulted in their enhanced ability to differentiate into adipocytes and osteocytes. Conclusions This protocol could be useful for maximizing h AT ‐ MSC potential to differentiate in vitro into the adipogenic and osteogenic lineages, for use in plastic and reconstructive surgery, and in tissue engineering strategies.