Open AccessImmobilization of thrombocytes on PCL nanofibres enhances chondrocyte proliferation in vitro
Author(s) -
Jakubova R.,
Mickova A.,
Buzgo M.,
Rampichova M.,
Prosecka E.,
Tvrdik D.,
Amler E.
Publication year - 2011
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/j.1365-2184.2011.00737.x
Subject(s) - chondrocyte , platelet , scaffold , polycaprolactone , in vitro , chemistry , biomedical engineering , tissue engineering , cell growth , materials science , platelet lysate , microbiology and biotechnology , nanotechnology , immunology , polymer , biology , biochemistry , medicine , composite material
Objectives: The aim of this study was to develop functionalized nanofibres as a simple delivery system for growth factors (GFs) and make nanofibre cell‐seeded scaffold implants a one‐step intervention. Materials and methods: We have functionalized polycaprolactone (PCL) nanofibres with thrombocytes adherent on them. Immobilized, these thrombocytes attached to nanofibre scaffolds were used as a nanoscale delivery system for native (autologous) proliferation and differentiation factors, in vitro . Pig chondrocytes were seeded on the thrombocyte‐coated scaffolds and levels of proliferation and differentiation of these cells were compared with those seeded on non‐coated scaffolds. Results: Immobilized thrombocytes on PCL nanofibres effectively enhanced chondrocyte proliferation due to time‐dependent degradation of thrombocytes and release of their GFs. Conclusions: These simply functionalized scaffolds present new possibilities for nanofibre applications, as smart cell scaffolds equipped with a GF delivery tool.