Open AccessCharacterization of immortalized mesenchymal stem cells derived from foetal porcine pancreas
Author(s) -
Cao H.,
Chu Y.,
Zhu H.,
Sun J.,
Pu Y.,
Gao Z.,
Yang C.,
Peng S.,
Dou Z.,
Hua J.
Publication year - 2011
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/j.1365-2184.2010.00714.x
Subject(s) - mesenchymal stem cell , islet , telomerase reverse transcriptase , immunocytochemistry , transplantation , pancreas , stem cell , biology , immortalised cell line , amniotic stem cells , microbiology and biotechnology , amniotic epithelial cells , cell culture , in vitro , adult stem cell , medicine , endocrinology , insulin , telomerase , endothelial stem cell , biochemistry , genetics , gene
Islet replacement therapy is limited by shortage of donor islet cells. Usage of islet cells derived from porcine pancreatic stem cells (PSCs) is currently viewed as the most promising alternative for human islet transplantation. However, PSCs are rare and have a finite proliferative lifespan. In this study, we isolated and established an immortalized mesenchymal stem cell (MSC) line derived from foetal porcine pancreas, by transfecting human telomerase reverse transcriptase (hTERT) and called these immortalized pancreatic mesenchymal stem cells (iPMSCs). The iPMSCs have been cultured for more than 80 passages and have capacity to differentiate into neurons, cardiomyocytes, germ cells and islet‐like cells, analysed by morphology, RT‐PCR, western blotting, immunofluorescence, immunocytochemistry and transplantation assay. Islets derived from iPMSCs reversed hyperglycaemia in streptozotocin‐induced diabetic mice and secreted insulin and C‐peptide in vitro . These results demonstrated that iPMSCs might provide unlimited resources for islet replacement therapy and models for functional cell differentiation.