Open AccessIn vitro ovine articular chondrocyte proliferation: experiments and modelling
Author(s) -
Mancuso L.,
Liuzzo M. I.,
Fadda S.,
Pisu M.,
Cincotti A.,
Arras M.,
La Nasa G.,
Concas A.,
Cao G.
Publication year - 2010
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/j.1365-2184.2010.00676.x
Subject(s) - petri dish , chondrocyte , in vitro , articular cartilage , biological system , flow cytometry , population , cell growth , cell , chemistry , biology , mathematics , microbiology and biotechnology , biomedical engineering , immunology , pathology , engineering , biochemistry , medicine , osteoarthritis , genetics , alternative medicine , environmental health
This study focuses on analysis of in vitro cultures of chondrocytes from ovine articular cartilage. Isolated cells were seeded in Petri dishes, then expanded to confluence and phenotypically characterized by flow cytometry. The sigmoidal temporal profile of total counts was obtained by classic haemocytometry and corresponding cell size distributions were measured electronically using a Coulter Counter. A mathematical model recently proposed (1) was adopted for quantitative interpretation of these experimental data. The model is based on a 1‐D (that is, mass‐structured), single‐staged population balance approach capable of taking into account contact inhibition at confluence. The model’s parameters were determined by fitting measured total cell counts and size distributions. Model reliability was verified by predicting cell proliferation counts and corresponding size distributions at culture times longer than those used when tuning the model’s parameters. It was found that adoption of cell mass as the intrinsic characteristic of a growing chondrocyte population enables sigmoidal temporal profiles of total counts in the Petri dish, as well as cell size distributions at ‘balanced growth’, to be adequately predicted.