Open AccessAntiproliferative effects of essential oils and their major constituents in human renal adenocarcinoma and amelanotic melanoma cells
Author(s) -
Loizzo M. R.,
Tundis R.,
Menichini F.,
Saab A. M.,
Statti G. A.,
Menichini F.
Publication year - 2008
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/j.1365-2184.2008.00561.x
Subject(s) - essential oil , eucalyptol , cytotoxic t cell , cell culture , chemistry , population , cytotoxicity , traditional medicine , biology , biochemistry , chromatography , in vitro , medicine , environmental health , genetics
. Objectives : The purpose of this study was to evaluate cytotoxic activity of Platycladus orientalis , Prangos asperula and Cupressus sempervirens ssp. pyramidalis essential oils and to identify active components involved in inhibition of population growth of human cancer cell lines. Materials and methods : Essential oils were obtained by hydrodistillation and were analysed by gas chromatography and gas chromatography coupled to mass spectrometry. Antiproliferative activity was tested on amelanotic melanoma C32 cells and on renal cell adenocarcinoma cells, using the sulphorhodamine B assay. Results : Cupressus sempervirens ssp. pyramidalis leaf oil exerted the highest cytotoxic activity with an IC 50 value of 104.90 µg/mL against C32, followed by activity of P. orientalis and P. asperula on the renal adenocarcinoma cell line (IC 50 of 121.93 and 139.17 µg/mL, respectively). P. orientalis essential oil was also active against amelanotic melanoma with an IC 50 of 330.04 µg/mL. Three identified terpenes, linalool, β‐caryophyllene and α‐cedrol, were found to be active on both cell lines tested. Conclusions : Our findings provide novel insights into the field of cytotoxic properties of essential oils. This study provided evidence on how cytotoxic activity of the oils is not always related to their major constituents, except for lower activity found in both cell lines for α‐cedrol. Interestingly, β‐caryophyllene and linalool exhibited comparable IC 50 values to the commercial drug vinblastine on the ACHN cell line. This opens a new field of investigation to discover mechanisms responsible for the observed activity.