Open AccessOn‐chip non‐invasive and label‐free cell discrimination by impedance spectroscopy
Author(s) -
SchadeKampmann G.,
Huwiler A.,
Hebeisen M.,
Hessler T.,
Di Berardino M.
Publication year - 2008
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/j.1365-2184.2008.00548.x
Subject(s) - flow cytometry , jurkat cells , cell , cell culture , microfluidics , cytometry , dielectric spectroscopy , in vitro , microbiology and biotechnology , cell type , biology , biomedical engineering , materials science , chemistry , nanotechnology , immunology , t cell , biochemistry , immune system , electrode , medicine , genetics , electrochemistry
. Objectives : Many flow‐cytometric cell characterization methods require costly markers and colour reagents. We present here a novel device for cell discrimination based on impedance measurement of electrical cell properties in a microfluidic chip, without the need of extensive sample preparation steps and the requirement of labelling dyes. Materials and methods, Results : We demonstrate that in‐flow single cell measurements in our microchip allow for discrimination of various cell line types, such as undifferentiated mouse fibroblasts 3T3‐L1 and adipocytes on the one hand, or human monocytes and in vitro differentiated dendritic cells and macrophages on the other hand. In addition, viability and apoptosis analyses were carried out successfully for Jurkat cell models. Studies on several species, including bacteria or fungi, demonstrate not only the capability to enumerate these cells, but also show that even other microbiological life cycle phases can be visualized. Conclusions : These results underline the potential of impedance spectroscopy flow cytometry as a valuable complement to other known cytometers and cell detection systems.