Open AccessInduction of pluripotent stem cells from mouse fibroblasts by four transcription factors
Author(s) -
Yamanaka S.
Publication year - 2008
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/j.1365-2184.2008.00493.x
Subject(s) - induced pluripotent stem cell , sox2 , embryonic stem cell , kosr , microbiology and biotechnology , biology , stem cell , klf4 , somatic cell , cell potency , embryoid body , induced stem cells , adult stem cell , regenerative medicine , homeobox protein nanog , transplantation , genetics , medicine , gene , surgery
. Pluripotent stem cells, such as embryonic stem cells (ESCs), proliferate rapidly while maintaining pluripotency, namely, the ability to differentiate into various types of cells. Embryonic stem cells are promising donor sources for cell transplantation therapies. However, human ESCs are also associated with ethical issues regarding the use of human embryos and rejection reactions after allogenic transplantation. It may be possible to overcome these issues by generating pluripotent stem cells directly from a patient's somatic cells. That somatic cell nuclei acquire an embryonic stem‐like status by fusion with ESCs suggests the existence of ‘pluripotency‐inducing’ factors. Previous studies have recently shown that retrovirus‐mediated transfection with four transcription factors (Oct‐3/4, Sox2, KLF4 and c‐Myc), which are highly expressed in ESCs, into mouse fibroblasts has resulted in generation of induced pluripotent stem (iPS) cells. iPS cells are similar to ESCs in morphology, proliferation, and pluripotency, judged by teratoma formation and chimaera contribution. If iPS cells can be derived from human somatic cells, then such cells may thus lead to important drug discoveries and advances in regenerative medicine.