Open AccessTime‐ and concentration‐dependent effects of resveratrol in HL‐60 and HepG2 cells
Author(s) -
Stervbo U.,
Vang O.,
Bonnesen C.
Publication year - 2006
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/j.1365-2184.2006.00406.x
Subject(s) - resveratrol , apoptosis , flow cytometry , cell cycle , ic50 , cell , cell culture , chemistry , cell growth , microbiology and biotechnology , biochemistry , in vitro , biology , genetics
. Resveratrol, a phytochemical present in grapes, has been demonstrated to inhibit tumourigenesis in animal models. However, the specific mechanism by which resveratrol exerts its anticarcinogenic effect has yet to be elucidated. In the present study, the inhibitory effects of resveratrol on cell proliferation and apoptosis were evaluated in the human leukaemia cell line HL‐60 and the human hepatoma derived cell line HepG2. We found that after a 2 h incubation period, resveratrol inhibited DNA synthesis in a concentration‐dependent manner. The IC 50 value was 15 µ m in both HL‐60 and HepG2 cells. When the time of treatment was extended, an increase in IC 50 value was observed; for example, at 24 h the IC 50 value was 30 µ m for HL‐60 cells and 60 µ m for HepG2 cells. Flow cytometry revealed that cells accumulated in different phases of the cell cycle depending on the resveratrol concentration. Furthermore, an increase in nuclear size and granularity was observed in the G 1 and S phases of HL‐60 treated and HepG2‐treated cells. Apoptosis was also stimulated by resveratrol in a concentration‐dependent manner in HL‐60 and HepG2 cells. In conclusion, resveratrol inhibits cell proliferation in a concentration‐ and time‐dependent manner by interfering with different stages of the cell cycle. Furthermore, resveratrol treatment causes stimulation of apoptosis as well as an increase in nuclear size and granularity.