Open AccessComparative analysis of proliferative potential and clonogenicity of MACS‐immunomagnetic isolated CD34 + and CD133 + blood stem cells derived from a single donor
Author(s) -
Freund D.,
Oswald J.,
Feldmann S.,
Ehninger G.,
Corbeil D.,
Bornhäuser M.
Publication year - 2006
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/j.1365-2184.2006.00386.x
Subject(s) - cd34 , progenitor cell , haematopoiesis , stem cell , immunomagnetic separation , biology , population , immunology , microbiology and biotechnology , chemistry , medicine , environmental health
. A novel stem cell marker prominin‐1 (CD133) has been shown to be expressed on a subpopulation of CD34 + haematopoietic stem and progenitor cells. The aim of this study was to compare in parallel commercially available CD34 + and CD133 + isolation methods based on paramagnetic bead‐coupled antibodies using clinical‐grade samples of mobilized peripheral blood from 10 individual healthy donors under identical conditions. The CD133 negative fraction from the first selection was used for CD34 + enrichment to obtain an additional CD34 + /CD133 − population. Although no significant difference in total cell expansion between cells isolated from the three procedures was observed in a 7‐day cytokine‐driven suspension culture, the long‐term culture‐initiating cell assay demonstrated that cells derived by CD34 + isolation contain less primitive progenitors than those isolated based on CD133 + selection. Interestingly, CD34 + ‐enriched progenitors, especially the CD34 + /CD133 − fraction, contained a significantly higher proportion of erythroid colony‐forming cells, whereas the highest content of myeloid colony‐forming cells was concentrated in the CD133 + selected cells. These subtle differences between CD34 + and CD133 + immunomagnetic selection will have to be explored for their potential clinical relevance.