Open AccessEffect of transfection with a gene coding for the fibronectin FNIII/10 fragment upon contact inhibition of C3H10T1/2 fibroblasts
Author(s) -
Miłoszewska J.,
Szaniawska B.,
Trembacz H.,
Gos M.,
Przybyszewska M.,
Swoboda P.,
Małecki M.,
Janik P.
Publication year - 2006
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/j.1365-2184.2006.00382.x
Subject(s) - transfection , fibronectin , cell culture , microbiology and biotechnology , contact inhibition , cell adhesion , gene , cell growth , biology , chemistry , cell , biochemistry , extracellular matrix , genetics
. The density‐dependent growth inhibition of non‐transformed cells may be associated with inefficient transduction of the proliferative signal from cell adhesion molecules. To verify this concept, the C3H10T1/2 fibroblasts were stably transfected with the gene coding for the fibronectin fragment III/10 (FNIII/10). This resulted in differences in gene's expression between original C3H10T1/2 cells and their FNIII/10 transfectants. No significant differences in growth properties were observed in the original or in the transfected cells. C3H10T1/2 cells and their transfectants, when co‐cultured, displayed more cells at confluence than the cells cultured alone. Moreover, co‐cultured C3H10T1/2 cells and their transfectants showed elevated levels of phospho‐ERK1/2 compared to homogenous cultures. Results obtained indicate that cellular homogeneity is responsible for density‐dependent growth inhibition.