Open AccessLack of synchrony among multiple nuclei induces partial DNA fragmentation in V79 cells polyploidized by demecolcine
Author(s) -
FujikawaYamamoto K.,
Ohdoi C.,
Yamagishi H.,
Zong Z. p.,
Murakami M.,
Yamaguchi N.
Publication year - 1999
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/j.1365-2184.1999.tb01352.x
Subject(s) - fragmentation (computing) , chinese hamster , microbiology and biotechnology , nucleus , biology , dna fragmentation , cell nucleus , cell culture , apoptosis , genetics , programmed cell death , ecology
. The nuclear morphology of polyploidized cells was examined in V79 Chinese hamster cells polyploidized by demecolcine or K‐252a, inhibitors of spindle fibre formation and protein kinases, respectively. A variety of nuclear morphologies, including multinuclei, were observed in V79 cells polyploidized by demecolcine but not by K‐252a, which produced mononuclear cells. A lack of synchrony in the nuclear cycle was observed among nuclei in multinuclear polyploidized cells. Partial DNA fragmentation, defined as DNA fragmentation of a nucleus in a multinuclear cell, was detected using the TUNEL method in V79 cells polyploidized by demecolcine but not by K‐252a. Apoptosis occurred earlier in cell populations treated with demecolcine than in these treated with K‐252a once the drugs were removed from the medium, suggesting that polyploidized cells with separate nuclei tend to apoptose earlier than those with mononuclei.