Open AccessThe antiproliferative activity of the tetrapeptide Acetyl‐N‐SerAspLysPro, an inhibitor of haematopoietic stem cell proliferation, is not mediated by a thymosin β4‐like effect on actin assembly
Author(s) -
Cheviron N.,
Grillon C.,
Carlier M.F.,
WdzieczakBakala J.
Publication year - 1996
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/j.1365-2184.1996.tb00986.x
Subject(s) - tetrapeptide , haematopoiesis , microbiology and biotechnology , biology , cell growth , cell cycle , stem cell , chemistry , cell , biochemistry , peptide
. Acetyl‐N‐SerAspLysPro (AcSDKP), known as a negative regulator of haematopoiesis, has been principally reported as an inhibitor of haematopoietic pluripotent stem cell proliferation. The tetrapeptide sequence is identical to the N‐terminus of thymosin β4 (Tβ4), from which it has been suggested that it may be derived. Recently, evidence was shown that Tβ4 plays a role as a negative regulator of actin polymerization leading to the sequestration of its monomeric form. The structural similarity between the N‐terminus of Tβ4 and AcSDKP has raised the possibility that AcSDKP may also participate in intracellular events leading to actin sequestration. The effect of Tβ4 on the proliferation of haematopoietic cells was compared to that of AcSDKP. The results revealed that Tβ4, like AcSDKP, exerts an inhibitory effect on the entry of murine primitive bone marrow cells into cell cycle in vitro . Qualitative electrophoretic analysis and quantitative polymerization assays were used to investigate the role of AcSDKP in actin polymerization. AcSDKP does not affect actin assembly at concentrations up to 50 μM, and does not compete with Tβ4 for binding to G‐actin. These results suggest that AcSDKP is not involved in cell cycle regulation via an effect on the process of actin polymerization.