Open AccessInfluence of long‐term limitation of cell proliferation on the cell cycle duration
Author(s) -
Blokhin A. V.,
Khalyavkin A. V.
Publication year - 1995
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/j.1365-2184.1995.tb00083.x
Subject(s) - mitosis , cell growth , cell cycle , microbiology and biotechnology , cell , senescence , biology , cell division , population , viability assay , cell culture , andrology , genetics , medicine , environmental health
The hypothesis was tested that constant, long‐term inhibition of cell proliferation is the primary cause of cellular senescence. Mouse L‐929 fibroblasts were maintained in confluent cultures for periods of 6 and 12 months with cell viability maintained by regular replacement of medium and serum. The mitotic activity of the cell population under these conditions was one mitosis per 10 4 cells. No changes in the duration of the cell cycle were observed when, following long‐term quiescence, the cell were replated and grown at low cell density. The results do not support the hypothesis that prolonged suppression of cell proliferation induces cell senescence.