Open AccessEffect of ebrotidine on the density of antral G‐cells in the gastric mucosa in the rat
Author(s) -
Romero A.,
Gömez F.,
Villamayor F.,
Sacristán A.,
Ortíz JA.
Publication year - 1995
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/j.1365-2184.1995.tb00080.x
Subject(s) - antrum , gastric mucosa , cimetidine , population , stomach , immunohistochemistry , medicine , gastroenterology , pathology , chemistry , environmental health
Two groups of male rats were treated orally for 60 days with ebrotidine and cimetidine, used as reference standard, respectively. The dose used of both drugs was 500mg/kg. A third group, used as control, received 10 ml/kg of an aqueous agar suspension. After receiving the last dose, the animals were killed by inhalation of CO 2 in a sealed chamber and the stomachs were removed for histological preparation. The purpose of this study was to count antral G‐cells throughout the gastric mucosa by light microscope. A PAP system‐associated antigastrin immunohistochemical method was used for cell identification. Cell density, with respect to their location in the gastric mucosa and the treatments given, was calculated using image analysis techniques. The results showed that ebrotidine did not cause any significant differences in the density of the population of these cells compared with the control group, while cimetidine induced a significant proliferation of antral G‐cells.