Open AccessLevels of selected growth factors in viable and necrotic regions of xenografted HCT‐8 human colon tumours
Author(s) -
Leith J. T.,
Michelson S.
Publication year - 1995
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/j.1365-2184.1995.tb00069.x
Subject(s) - necrosis , biology , growth factor , platelet derived growth factor , transforming growth factor , platelet derived growth factor receptor , fibroblast growth factor , vascular endothelial growth factor , pathology , basic fibroblast growth factor , cell , fibroblast , vegf receptors , cell culture , cancer research , endocrinology , medicine , biochemistry , receptor , genetics
Xenografted tumours were produced in nude mice by injection of HCT‐8 human colon tumour cells. At average volumes of about 750 mm 3 , animals were injected with fast green vital dye, and 20 min later, tumours were excised and dissected into viable (stained) and necrotic portions (unstained). Viable and necrotic regions were then examined for cell yields, colony forming efficiencies, and levels of basic fibroblast growth factor (FGF‐2), transforming growth factors‐β 1 and ‐α (TGF‐β 1 , TGF‐α), platelet derived growth factor (PDGF), and vascular endothelial growth factor (VEGF) using enzyme‐linked immunoassay (ELISA) procedures. Levels in the viable and necrotic regions were compared to levels in unseparated tumours. The average extent of necrosis in HCT‐8 tumours of this size was 64%. The data for cell yields, colony forming efficiencies FGF‐2, VEGF, TGF‐β 1 and TGF‐α indicated that values determined in the unseparated tumours could be understood on the basis of the weighted average between viable and necrotic tissue, with the higher values occurring in the viable tissue. Low levels of FGF‐2 and VEGF were found in the necrotic portions of the tumour while no measurable levels of TGF‐β 1 and TGF‐α could be determined. PDGF levels were, however, equivalent in both the viable and necrotic regions indicating that necrotic tissue could be an important reservoir for this growth factor.