Open AccessRegulation of thymosin β4 mRNA levels during cell proliferation
Author(s) -
Zalvide J. B.,
Alvarez C. V.,
Vidal A.,
Dieguez C.,
Vega F. V.,
Domínguez F.
Publication year - 1995
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/j.1365-2184.1995.tb00057.x
Subject(s) - thymosin , messenger rna , microbiology and biotechnology , cell growth , cell , biology , immunology , chemistry , biochemistry , gene
The levels of thymosin β4 mRNA were studied throughout the cell cycle of NIH 3T3 cells. In serum deprived, quiescent cells, the levels of thymosin β4 were undetectable; after serum restoration, the cells were induced to proliferate and we found a pronounced increase in thymosin β4 mRNA levels at the G 1 /S transition. Thymosin β4 mRNA was induced even in the presence of cycloheximide. On the other hand, cycling cells that were synchronized at different stages of the cycle by means of mitotic shake‐off after nocodazole arrest or a double thymidine block did not show any variation in the levels of thymosin β4 mRNA when they progressed synchronously through the cycle. In conclusion, the present data indicate that the thymosin β4 gene is regulated by cell proliferation but it is not a cell cycle‐regulated gene. Finally, we studied thymosin β4 mRNA stability by inhibiting thymosin β4 gene transcription with actinomycin D. Our results suggest that thymosin β4 mRNA has a pronounced stability, a fact that might be relevant to account for the presence of thymosin β4 in enucleated cells like platelets.