Open AccessChanges in oncogene expression in ascite tumour cells during ageing
Author(s) -
Biagetti M.,
Fazia M. A. Della,
Servillo G.,
ViolaMagni M. P.
Publication year - 1994
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/j.1365-2184.1994.tb01416.x
Subject(s) - oncogene , flow cytometry , immunofluorescence , biology , northern blot , western blot , microbiology and biotechnology , cell cycle , messenger rna , antibody , gene expression , cancer research , cell , gene , immunology , genetics
The expression of two oncogenes, c‐myc and c‐fos , was studied in an ascitic tumour (ATPC+) at different times after implantation. The specific mRNA synthesis was analysed by Northern blot analysis. The presence of the oncogene proteins was shown by immunofluorescence using flow cytometry and referred to the distribution of the cells in the different cell phases. The results show that both oncogenes are expressed by ATPC+ tumour cells. c‐my is expressed 5, 8 and 12 days after implantation, although with a different intensity, and the protein is mainly present in S or S+G 2 phase cells. The c‐fos oncogene is expressed only 12 days after tumour implantation and the cells labelled with the specific antibody are mainly in G 1 phase. We conclude that c‐myc is principally correlated with proliferative activity, whereas c‐fos is expressed by non‐cycling cells.