Open AccessA comparison of different methods to determine cell proliferation by flow cytometry
Author(s) -
Zölzer F.,
Streffer C.,
Pelzer T.
Publication year - 1994
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/j.1365-2184.1994.tb01383.x
Subject(s) - flow cytometry , proliferating cell nuclear antigen , cell cycle , labelling , cell growth , biology , microbiology and biotechnology , in vitro , cell , cytometry , dna synthesis , biochemistry
. The proliferation of human melanoma cells (MeWo) in vitro was studied with a number of different techniques. In particular, we compared the expression of PCNA and the Ki‐67 antigen on the one hand with BrdU pulse and continuous labelling on the other. Two‐dimensional flow cytometry (with DNA content as a second parameter) was employed to discriminate between cycling and non‐cycling cells as well as cells in the G 1 , S and G 2 phases of the cycle. Cell cultures in different stages of growth were analyzed. We found that the percentage of anti‐PCNA and Ki‐67 positive cells agreed very well with the BrdU pulse and continuous labelling index, respectively. Our data further support the assumption that under certain conditions PCNA is a marker of S‐phase cells, whereas Ki‐67 can be used to quantify the growth fraction. Possible pitfalls of the techniques are discussed.