Open AccessIntratumoral heterogeneity of S phase transition in solid tumours determined by bromodeoxyuridine labelling and flow cytometry
Author(s) -
Baisch H.,
Otto U.
Publication year - 1993
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/j.1365-2184.1993.tb00131.x
Subject(s) - labelling , flow cytometry , propidium iodide , bromodeoxyuridine , fluorescein isothiocyanate , chemistry , kinetics , microbiology and biotechnology , cell cycle , cell , biology , fluorescence , cell growth , biochemistry , physics , apoptosis , programmed cell death , quantum mechanics
. Cell kinetics of human renal cell carcinomas xenotransplanted into nu/nu mice were analysed using the bromodeoxyuridine (BrdUrd) labelling method. Tumours were removed 0.5–14 h after injection of the BrdUrd solution. The tumour cells were stained with fluorescein isothiocyanate conjugated anti‐BrdUrd antibodies and propidium iodide (DNA content). From the flow cytometry data the relative movement was calculated. Relative movement data of variable intervals after BrdUrd labelling were subjected to a fit procedure using log‐normal distributions for S phase transition (T s ). The log‐normal distributions were modified by inflation factors in order to get extremely asymmetric distributions. The best fits to the experimental data were obtained using wide asymmetric T s distributions, indicating that progression through S phase in solid human tumours is considerably heterogeneous. This implies that the potential doubling time (T pot ) is longer than calculated from a single measured relative movement value obtained a few hours after BrdUrd labelling.