Open AccessProliferating cell nuclear antigen and Ki‐67 antigen expression in human haematopoietic cells during growth stimulation and differentiation
Author(s) -
Landberg* G.,
Roos G.
Publication year - 1993
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/j.1365-2184.1993.tb00130.x
Subject(s) - proliferating cell nuclear antigen , cell cycle , antigen , haematopoiesis , biology , microbiology and biotechnology , cell growth , flow cytometry , cell , immunology , stem cell , biochemistry
. By flow cytometric dual parameter analysis of proliferating cell nuclear antigen (PCNA) and the Ki‐67 antigen a detailed cell cycle analysis can be performed. In this study the co‐ordinated expression of these two growth‐related antigens was investigated in human haematopoietic cells at entrance into the cell cycle as well as at exit from the cycle. In mitogen‐stimulated peripheral blood lymphocytes entering the first cell cycle, the Ki‐67 antigen was found to be expressed in S phase cells and not in G 1 cells. Thus, the Ki‐67 antigen expression in PCNA‐positive S phase cells differed between continuously cycling cells and cells entering the cell cycle. Based on this difference, it was possible to visualize and evaluate the recruitment of cells into the first cell cycle from a resting stage. This new cell cycle parameter can give additional information concerning tumour growth. The Ki‐67 antigen was also studied during different stages of G 1 and was found to be expressed at high levels in early G 1 cells compared with other parts of G 1 .