Open AccessCell cycle analysis of asexual stages of erythrocytic malaria parasites
Author(s) -
Jacobberger J. W.,
Horan P. K.,
Hare J. D.
Publication year - 1992
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/j.1365-2184.1992.tb01452.x
Subject(s) - schizogony , biology , parasite hosting , malaria , dna , cell cycle , red blood cell , plasmodium (life cycle) , flow cytometry , microbiology and biotechnology , virology , genetics , cell , plasmodium falciparum , immunology , world wide web , computer science
. Intra‐erythrocytic Plasmodium species can be stained with the DNA binding dye, Hoechst 33342, and the distribution of DNA content determined for parasite populations by flow cytometric measurement of fluorescence. Analysis of this distribution will determine the parasitaemia (percentage of erythrocytes infected), and the percentages of trophozoite infected red blood cells, polyparasitized (trophozoite) red blood cells, and schizont/segmenter infected red blood cells. This analysis is based on the hypothesis that the asexual parasites cycle with single G 1 period, and effectively, a single S phase with no significant G 2 /M period except at schizogony when the genome DNA content is equivalent to 8 N or higher, dependent on the species. Data are presented to support this model.