Open AccessAltered proliferative kinetics in PHA‐activated human T‐lymphocytes treated with the anti‐HLA class I monoclonal antibody 01.65
Author(s) -
Rubini M.,
Selvatici R.,
Orlando P.,
Balugani S.,
Balboni A.,
Gandini E.
Publication year - 1992
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/j.1365-2184.1992.tb01450.x
Subject(s) - monoclonal antibody , human leukocyte antigen , t lymphocyte , microbiology and biotechnology , immunology , antibody , biology , monoclonal , lymphocyte , kinetics , chemistry , antigen , physics , quantum mechanics
. Anti‐HLA class I monoclonal antibody (mAb) 01.65 inhibited phyto‐haemagglutinin (PHA)‐induced human lymphocyte proliferation. The inhibitory effect was inversely correlated to the strength of the proliferative response. It was increased when lymphocytes were stimulated with suboptimal doses of PHA but it disappeared with supraoptimal doses. Proliferation inhibition was achieved by prolonging the cell cycle time and by slowing down its recruitment rate. The former effect was not restricted to the G 1 ‐phase but also included the S phase. These results support the idea that HLA class I molecules are important in the PHA‐induced proliferation of human T‐lymphocytes.