Open AccessChanges in cell cycle and chromatin distribution in C6 glioma cells treated by dibutyryl cyclic AMP
Author(s) -
Mareš V.,
Giordano P. A.,
Pellicciari C.,
Scherini E.,
Lisà V.,
Bottone M. G.,
Bottiroli G.
Publication year - 1991
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/j.1365-2184.1991.tb01185.x
Subject(s) - chromatin , distribution (mathematics) , cell cycle , microbiology and biotechnology , glioma , cell , chemistry , biology , cancer research , biochemistry , dna , mathematics , mathematical analysis
. C6 glioma cells in culture were treated with 1 mm dibutyryl cyclic AMP (Db‐cAMP) for 5,8 ,24 and 72 h. The cells were labelled with [ 3 H]‐thymidine before either the end, or the beginning, of the Db‐cAMP treatment. The cell cycle passage was monitored by the simultaneous determination of DNA content and DNA synthesis in propidium iodide stained autoradiograms. The data revealed an early ( t ≤ 3–8 h) and moderate inhibitory effect of Db‐cAMP on all phases of the cell cycle except mitosis; some cells (2%) were completely blocked in the S phase. Later (8 < t < 24–72 h), the cycling of a substantial part of the population became inhibited in G 1 phase. Microdensitometric texture analysis of Feulgen‐stained nuclei, performed 24 h after administration of Db‐cAMP, showed a higher inhomogeneity of the DNA distribution in cell nuclei, caused by the condensation of a part of the chromatin. This may reflect either changes in genome expression taking part in the process of cAMP induced differentiation or transit of some cells into quiescent G 0 or S 0 phases.