Open AccessStimulator of proliferation of spleen colony‐forming cells in T‐cell deprived mice treated with cyclophosphamide or irradiation
Author(s) -
Milenković P.,
Ivanović Z.,
Kataranovski M.,
Lukić M. L.
Publication year - 1991
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/j.1365-2184.1991.tb01178.x
Subject(s) - bone marrow , cyclophosphamide , spleen , cell growth , biology , immunology , andrology , medicine , chemotherapy , biochemistry
. A role for T‐cells in the regulation of CFU‐S proliferation was investigated by determining the presence and activity of CFU‐S proliferation stimulator (CFU‐S stimulator) in adult mouse bone marrow after irradiation or cyclophosphamide (Cy) treatment. CBA mice previously deprived of T‐cells by thymectomy, irradiation and bone marrow reconstitution (TIR) were thereafter treated with 4·5 Gy irradiation or 200 mg/kg Cy. Regenerating bone marrow cells of TIR and corresponding control mice after irradiation or Cy treatment produced CFU‐S stimulator. The dose dependent increase in cytosine arabinoside cell death of normal bone marrow day 8 CFU‐S was found when both CFU‐S stimulators obtained after irradiation of TIR or corresponding control animals were tested. CFU‐S stimulator activity in the bone marrow of TIR‐Cy treated mice was also detected, but the effect was not dose‐dependent. This was not related to the presence of an inhibitor of CFU‐S proliferation. It appears that the CFU‐S stimulator activity is not related to IL‐6, IL‐1 or IL‐2, or to an inhibitor of IL‐6 or IL‐1 activity. The results demonstrate the existence of CFU‐S proliferation stimulator unrelated to the two major monokines in the bone marrow of immunosuppressed mice.