Open AccessGrowth and cell kinetics of human hair papilla cells in vitro . An autoradiographic and flow cytometric study
Author(s) -
Bassukas I. D.,
Arai A.,
Schell H.,
Hornstein O. P.
Publication year - 1991
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/j.1365-2184.1991.tb01165.x
Subject(s) - doubling time , flow cytometry , subculture (biology) , biology , cell growth , hair follicle , cell cycle , microbiology and biotechnology , cell division , in vitro , kinetics , cell , biochemistry , botany , physics , quantum mechanics
. Hair papilla, a distinct specialized dermal compartment, plays a fundamental role in the biology of hair growth. Recently some attention has been focused on hair papilla cells (HPC) as possible targets for drugs influencing the hair growth. Isolation and cultivation of the HPC facilitates screening for such drugs. In the present work, growth and cell kinetics of human occipital scalp follicle HPC have been studied in vitro . HPC grow according to a Gompertz function, i.e. with considerable growth delay long before becoming confluent cultures, due probably to elongation of the potential doubling time (T pot ) and to a parallel increasing cell loss rate. The [ 3 H]dT labelling index of the HPC strongly depends on the age of the subculture; the cycle time being about 4 days. A potential doubling time of about 93 h, indicative of growth fraction (GF) = 1 , and a duration of S phase and G 2 + M phase of about 8 h each were found by the combined application of continuous labelling with [ 3 H]dT and DNA flow cytometry.