Open AccessThe effect of chemical radiation protectors on cell cycle progression after gamma or neutron irradiation
Author(s) -
Sigdestad C. P.,
Bergquist B. L.,
Grdina D. J.
Publication year - 1991
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/j.1365-2184.1991.tb01156.x
Subject(s) - irradiation , chinese hamster , radiochemistry , dapi , gamma irradiation , neutron , neutron irradiation , cell cycle , chemistry , relative biological effectiveness , nuclear medicine , biophysics , biology , cell , biochemistry , dna , medicine , physics , nuclear physics , apoptosis
. The effects of two chemical radiation protectors, WR‐1065 and WR‐151326, were characterized in V79 Chinese hamster cells after either cobalt‐60 ( 60 Co) gamma or fission spectrum neutron irradiation. Each protector was administered at a concentration of 4 mM to exponentially growing cultures for 30 min prior to and during irradiation with either 60 Co gamma or JANUS fission spectrum neutrons. After irradiation the cells were either plated immediately for survival or returned to the incubator and assayed for cell progression. Aliquots of cells were removed at selected times, counted, fixed and stained with 4'6‐diamidino‐2‐phenylindole (DAPI). Analysis of DNA histograms indicate that the presence of the protector during irradiation reduced the division delay experienced at the G 2 ‐M interface. Implications of these effects are discussed.