Open AccessKinetics of the development of accelerated cell‐cycle transit resulting from inhibition of DNA replication in the previous cycle
Author(s) -
Tolmach L. J.,
Labanowska J.
Publication year - 1990
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/j.1365-2184.1990.tb01339.x
Subject(s) - aphidicolin , dna synthesis , dna replication , cell cycle , hela , dna , microbiology and biotechnology , biology , chemistry , cell , biochemistry
. Shortening of the generation cycle in cells in which DNA synthesis had been temporarily inhibited in the previous generation, which has been reported several times in recent years, has been confirmed in HeLa cells. As in the previous studies, the shortening is attributable to accelerated transit of G 1 resulting from the accumulation, during the inhibition, of a factor needed for initiation of DNA replication. It is shown that partial (85–96%) inhibition with any one of three inhibitors is effective when the inhibitor is added in G 1 or in S, but more complete (99%) inhibition is effective only if the inhibitor is added after cells have entered S. In addition, cells begin to respond to the inhibition after a lag that increases as DNA synthesis in the early part of S is progressively inhibited with aphidicolin, indicating that competence to respond is achieved only after cells have reached a particular point in the replication of their genome.