Open AccessIn vivo effect of the tetrapeptide, N‐Acetyl‐Ser‐Asp‐Lys‐Pro, on the G 1 ‐S transition of rat hepatocytes
Author(s) -
Lombard M.N.,
Sotty D.,
WdzieczakBakala J.,
Lenfant M.
Publication year - 1990
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/j.1365-2184.1990.tb01336.x
Subject(s) - tetrapeptide , in vivo , haematopoiesis , heterologous , hepatocyte , in vitro , biology , microbiology and biotechnology , chemistry , stem cell , biochemistry , peptide , genetics , gene
. The synthetic molecule N‐Acetyl‐Ser‐Asp‐Lys‐Pro (Ac‐SDKP), corresponding to the low molecular weight inhibitory factor preventing in vivo haematopoietic stem cell (CFU‐S) entry into DNA synthesis, was tested in two heterologous systems in vivo: adult regenerating rat liver and 10‐day‐old rat hepatocytes synchronized by an irritating trigger. In both systems, it was shown that doses of 2–8 μg kg ‐1 of tetrapeptide inhibited 50–70% of the hepatocyte G 1 ‐S transitions.