Open AccessDifferent nucleolar antigen expression in resting and proliferating human lymphocytes as studied by fluorescence microscopy and flow cytometry
Author(s) -
Dubben H.H.
Publication year - 1990
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/j.1365-2184.1990.tb01335.x
Subject(s) - nucleolus , immunofluorescence , propidium iodide , flow cytometry , biology , antigen , microbiology and biotechnology , fluorescence microscope , pathology , phytohaemagglutinin , immunostaining , nucleolus organizer region , antibody , fluorescence , lymphocyte , immunology , immunohistochemistry , cytoplasm , apoptosis , medicine , biochemistry , physics , quantum mechanics , programmed cell death
. The aim of this study was to investigate the use of a nucleolar antigen to discriminate between proliferating and resting cells. Antinucleolar antibodies (Si87) were obtained from a scleroderma patient. The specificity of immunostaining was verified and morphological changes in nucleoli were monitored using a fluorescence microscope. Fluorescence of propidium iodide‐stained DNA and nucleolar immunofluorescence were measured by flow cytometry. Following phytohaemagglutinin stimulation the number of nucleoli of normal human peripheral blood lymphocytes increased about 3‐fold, accompanied by enlargement of nucleolar size. Simultaneously a mean increase in total immunofluorescence per cell by a factor of three was detected. The method developed and applied here allows a discrimination between resting and proliferating human lumphocytes on the basis of their nucleolar antigen content.