Open AccessLocal regulation of haemopoietic stem cell proliferation in mice following irradiation
Author(s) -
Ali A. M.,
Wright E. G.,
Riches A. C.
Publication year - 1989
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/j.1365-2184.1989.tb00218.x
Subject(s) - bone marrow , irradiation , cfu gm , cell growth , cell cycle , stem cell , in vitro , spleen , biology , colony forming unit , cell , andrology , immunology , microbiology and biotechnology , haematopoiesis , medicine , biochemistry , physics , nuclear physics , genetics , bacteria
Changes in the kinetic state of pluripotent haemopoietic spleen colony forming cells (CFU‐S) and of the CFU‐S proliferation stimulator have been studied following whole‐body X‐irradiation. Rapid recruitment of CFU‐S into cell cycle by 30 min after irradiation was observed following low doses (0.5 Gy) but a delay of 6 h occurred after higher doses (1.5 and 4.5 Gy). These changes in proliferative state correlated with the presence of the CFU‐S proliferation stimulator. CFU‐S irradiated in vitro in bone marrow plugs were also recruited into cycle illustrating directly the local nature of the feedback mechanism. CFU‐S removed from 1.5 Gy irradiated recipients at a time when they were not in cycle were not responsive to the CFU‐S proliferation stimulator. The CFU‐S proliferation stimulator was produced by Ia positive cells in the irradiated bone marrow. The regulation changes occurring shortly after irradiation cannot simply be controlled by the size of the CFU‐S compartment.