Open AccessDNA synthesis in Langerhans’cells of mouse ear epithelium revealed by tritiated thymidine autoradiography and histochemical staining for non‐specific esterase and beta‐glucuronidase activity
Author(s) -
Hume W. J.,
Moore J. K.
Publication year - 1989
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/j.1365-2184.1989.tb00216.x
Subject(s) - thymidine , staining , esterase , biology , epithelium , microbiology and biotechnology , beta glucuronidase , dna , enzyme , dna synthesis , biochemistry , gene expression , genetics , gene
The proportion of Langerhans’cells in DNA synthesis in normal mouse skin was assessed by combining tritiated thymidine [ 3 H]TdR autoradiography with enzyme histochemistry. After injection of [ 3 H]TdR, ear skin was treated in two ways. Epithelial sheet preparations were stained for the presence of non‐specific esterase and cytospin preparations of epithelial cell suspensions were stained for beta‐glucuronidase activity. The labelling index (p± SE mean) for cytospins, 40 min after injecting [ 3 H]TdR, was 1.6 ± 0.15%, doubling to 3–4% from 7–17 days after injection. The sheet preparations showed the proportion of label attributable to paired Langerhans’cells rising from 18% at 40 min after injection, to approximately 45%, on days 1–4 after injection. These results suggest that the proliferation of Langerhans’cells in normal mouse skin might be higher than was previously thought to be the case.