Open AccessHaemopoietic Inductive Capacity of Irradiated Stromal Cell Layers In Human Micro Long‐Term Bone Marrow Cultures
Author(s) -
Brühl P.,
Mergenthaler H.G.,
Dörmer P.
Publication year - 1988
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/j.1365-2184.1988.tb00801.x
Subject(s) - stromal cell , bone marrow , haematopoiesis , cfu gm , cell culture , in vitro , colony forming unit , cell , irradiation , biology , immunology , chemistry , stem cell , microbiology and biotechnology , andrology , cancer research , medicine , biochemistry , genetics , physics , bacteria , nuclear physics
. In a micro long‐term bone marrow culture (LTBMC) system the effects of irradiation on confluent stromal cell layers were studied. In order to individually analyse the number of granulocyte‐macrophage colony‐forming cells (GM‐CFC) per LTBMC a miniaturized human GM‐CFC assay was established. the normalized GM‐CFC numbers in the micro‐assay compared well with data by the conventional GM‐CFC assay. Pre‐formed stromal cell layers were irradiated with doses up to 20 Gy and subsequently recharged with allogeneic bone marrow cells (BMC). Immediately before recharge the BMC were stromal cell‐depleted by nylon wool filtration. When stromal cell‐depleted BMC were inoculated on empty culture dishes, in vitro haemopoiesis rapidly declined. Sustained GM‐CFC production, however, was seen when these cells were used as a second inoculum. It is concluded that irradiation doses of up to 20 Gy do not cause alteration of the haemopoietic inductive capacity of confluent stromal cell layers.