Open AccessDouble Labelling With Bromodeoxyuridine and ( 3 H)‐Thymidine of Proliferative Cells In Small Intestinal Epithelium In Steady State and After Irradiation
Author(s) -
Chwalinski S.,
Potten C. S.,
Evans G.
Publication year - 1988
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/j.1365-2184.1988.tb00790.x
Subject(s) - crypt , bromodeoxyuridine , thymidine , ileum , biology , cell cycle , epithelium , cell division , microbiology and biotechnology , small intestine , cell growth , labelling , immunohistochemistry , intestinal epithelium , cell , biochemistry , immunology , dna , endocrinology , genetics
. The simultaneous immunohistochemical detection of bromodeoxyuridine (BrdU) and [ 3 H]‐thymidine ([ 3 H]TdR), by conventional autoradiography, was performed on the mouse small intestine (ileum). Proliferation was studied under normal conditions as well as after 3 Gy of γ‐rays. the BrdU method in conjunction with [ 3 H]TdR autoradiography appears to be reliable and useful for the study of cell kinetics especially in disturbed states, on condition that [ 3 H]TdR is delivered to the animals before BrdU. It has been found that cells in the crypt are delayed by irradiation in their progression through the cell cycle predominantly in late S phase. the cells at the bottom of the crypt are more affected than the more differentiated but proliferating cells in the upper part of the crypt.