Open AccessGrowth of Im‐9 Human Lymphoblasts In Serum‐Free Medium: Stimulation By Glucocorticoids
Author(s) -
Straus D. S.
Publication year - 1988
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/j.1365-2184.1988.tb00777.x
Subject(s) - endocrinology , medicine , aldosterone , potency , lymphoblast , stimulation , chemically defined medium , transferrin , receptor , biology , hormone , bovine serum albumin , hydrocortisone , chemistry , cell culture , in vitro , biochemistry , genetics
. The IM‐9 human B‐lymphoblast cell line grows well in a completely defined serum‐free medium containing insulin, transferrin, low density lipoprotein and oleic acid in complex with fatty acid‐free bovine serum albumin. Growth of the IM‐9 cells is stimulated by addition of physiological concentrations of hydrocortisone to this medium. the order of growth stimulatory potency of several steroids is dexamethasone > hydrocortisone > aldosterone, whereas testosterone does not stimulate growth of the IM‐9 cells. This order of potency suggests that the effect is mediated by binding to glucocorticoid receptors. Growth of the IM‐9 cells is also stimulated by the neuropeptide substance P. the defined serum‐free medium described in this report will be useful for further studies of the biological responses of the IM‐9 cells to other hormones in the absence of interference from hormones and growth factors present in serum.