Open AccessThe Spleen Colony Technique. I. Correction For the Overlap Effect and Sources of Error In Cfu‐S Determination
Author(s) -
Znojil V.,
Nečas E.
Publication year - 1988
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/j.1365-2184.1988.tb00772.x
Subject(s) - spleen , systematic error , biology , biological system , immunology , statistics , mathematics
Abstract. A linear model for the errors of the ‘spleen colony’ assay for haemopoietic stem cells has been derived. the components emerging from the model are interpreted and practical recommendations given for interpreting measurements made with this assay. the model permits correction for the effect of overlapping colonies and gives average errors for single measurements of the number of CFU‐s. More reliable and more precise information can be obtained using this model.