Open AccessEvidence for arrested G 2 cell subpopulation in rat liver inducible to mitosis
Author(s) -
GómezLechón J.,
Castell J. V.
Publication year - 1987
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/j.1365-2184.1987.tb01367.x
Subject(s) - mitosis , hepatocyte , xenobiotic , biology , cell cycle , thymidine , cell , microbiology and biotechnology , chemistry , biochemistry , dna , enzyme , in vitro
The intraperitoneal administration of several substances (biliverdin, heat‐killed bacteria and diatomaceous earth) to rats caused the prompt appearance of a mitotic wave in the liver. Autoradiographic analysis of livers of treated animals showed no evidence of [ 3 H]‐thymidine uptake by mitotic hepatocytes. In addition, livers from xenobiotic‐treated rats showed a very low thymidine kinase activity, close to that found in normal livers. This excludes the possibility that non‐cycling cells move to mitosis through the S phase. The results suggest that mitosis could be derived from a hepatocyte subpopulation arrested in the G 2 phase of the cell cycle, which is stimulated to divide by the xenobiotics.