Open AccessCell cycle responses of heterogeneous human colon adenocarcinoma subpopulations to X‐irradiation
Author(s) -
Bliven S. F.,
Schneiderman T. E.,
Leith J. T.
Publication year - 1987
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/j.1365-2184.1987.tb01356.x
Subject(s) - clone (java method) , flow cytometry , elutriation , cell cycle , cell , biology , cell culture , irradiation , microbiology and biotechnology , chemistry , physics , genetics , gene , organic chemistry , nuclear physics
The cell cycle responses of two exponentially growing subpopulations of cells (clones A and D), originally obtained from a human colon adenocarcinoma to X‐irradiation, were studied using centrifugal elutriation. Cell suspensions were separated by changing counter‐current flow rate while keeping the rotor speed constant (1600 rpm) and the composition of eluted fractions was determined using flow cytometry. The X‐ray sensitivity of unseparated clone D cells was somewhat greater than that of clone A cells (e.g. 10% greater at the 10% level of survival). This difference appeared to be due to a greater value of the α parameter (one‐hit cell killing), using the linear‐quadratic equation in which the relative survival S / S 0 = exp – (αD +βD 2 ) with dose (D) in Gy. This finding was confirmed in the cell cycle studies where the α parameter was always greater for the clone D cells than for the clone A cells. The β parameter was essentially the same for both cell lines through the cell cycle.