Open AccessVariability of ecdysteroid‐induced cell cycle alterations in Drosophila Kc sublines
Author(s) -
Besson M. T.,
Cordier G.,
Quennedey B.,
Quennedey A.,
Delachambre J.
Publication year - 1987
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/j.1365-2184.1987.tb01326.x
Subject(s) - ecdysteroid , clone (java method) , biology , cell cycle , cell culture , microbiology and biotechnology , moulting , cell , 20 hydroxyecdysone , hormone , medicine , endocrinology , genetics , botany , dna , larva
. The cell cycle of two lines isolated from Drosophila Kc cells was followed by flow cytofluorometry and cell counting. The first line is the 8‐9K clone which grew in a medium supplemented with 5% serum; the second, named subline Kc0, grew in a serum‐free medium. The stationary phase is characterized by a G 2 cell accumulation: 73% in the 8‐9K clone and 50% in the Kc0 subline. When the medium was supplemented with the steroid moulting hormone 20‐hydroxyecdysone, more than 90% of 8‐9K cells and 65% of Kc0 cells were progressively arrested in G 2 . In the continuous presence of 20‐hydroxyecdysone, most of the 8‐9K cells remain G 2 ‐arrested; no massive G 2 release into M was observed and only a few cells were able to divide. When treated for only 3 or 7 days, a transient release into M and proliferation occurred after hormone‐free medium renewal, largely masked by G 2 cell death. These results are discussed in comparison with other reports on cell cycle alteration induced by ecdysteroids.