Open AccessSimultaneous staining of exponentially growing versus plateau phase cells with the proliferation‐associated antibody Ki‐67 and propidium iodide: analysis by flow cytometry
Author(s) -
Baisch H.,
Gerdes J.
Publication year - 1987
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/j.1365-2184.1987.tb01323.x
Subject(s) - propidium iodide , flow cytometry , staining , microbiology and biotechnology , biology , cell cycle , bromodeoxyuridine , serial dilution , cell growth , antibody , chemistry , immunology , cell , apoptosis , pathology , medicine , biochemistry , programmed cell death , genetics , alternative medicine
. The antibody Ki‐67, which detects proliferating cells, was used in combination with propidium iodide, a DNA‐specific dye. The double‐staining method allowed discrimination of cells in the phases of the cell cycle as well as the recognition of Ki‐67 staining characteristics. Suspension cultures of U937 cells were measured in exponential growth and plateau phase in nutritional deprivation. The fraction of Ki‐67 positive cells was nearly 100% 2 days after dilution and 46% 7 days after dilution of the cultures. Stathmokinetic measurements with colchicine and flow cytometry measurements with the BrdU‐Hoechst technique yielded close to 100% proliferation at day 2 but only 18% and 6%, respectively, at day 7. The discrepancy between Ki‐67 results and the results of the two other methods is considered to be a characteristic of nutritionally deprived cells.